Document Type
Article
Publication Date
2019
Publication Title
Journal of Cell Science
Volume
132
Abstract
Adipocyte functionality, including adipocyte differentiation and adipokine secretion, is essential in obesity-associated metabolic syndrome. Here, we provide evidence that Ca2+ influx in primary adipocytes, especially upon store-depletion, plays an important role in adipocyte differentiation, functionality, and subsequently metabolic regulation. The endogenous Ca2+ entry channel in both subcutaneous and visceral adipocytes was dependent on TRPC1-STIM1 and blocking Ca2+ entry with SKF-96365 or TRPC1-/- derived adipocytes inhibited adipocyte differentiation. Additionally, TRPC1-/- mice have decreased organ weight, but increased adipose deposition and reduced serum adiponectin and leptin concentrations, without affecting total adipokine expression. Mechanistically, TRPC1- mediated Ca2+ entry regulated SNARE complex formation and agonist –mediated secretion of adipokine loaded vesicles was inhibited in TRPC1-/- adipose. These results suggest an unequivocal role of TRPC1 in adipocytes differentiation and adiponectin secretion, and loss of TRPC1 disturbs metabolic homeostasis.
Issue
13
DOI
10.1242/jcs.231878
ISSN
00219533
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Thad A. Rosenberger, Anne Schaar, Yuygang Sun, et al.. "Ca2+ Entry via TRPC1 is Essential for Cellular Differentiation and Modulates Secretion via the SNARE Complex" (2019). Biomedical Sciences Faculty Publications. 11.
https://commons.und.edu/bms-fac/11