Document Type
Article
Publication Date
12-3-2019
Publication Title
Scientific Data
Volume
6
Abstract
Despite the use of Hymenolepis diminuta as a model organism in experimental parasitology, a full genome description has not yet been published. Here we present a hybrid de novo genome assembly based on complementary sequencing technologies and methods. The combination of Illumina pairedend, Illumina mate-pair and Oxford Nanopore Technology reads greatly improved the assembly of the H. diminuta genome. Our results indicate that the hybrid sequencing approach is the method of choice for obtaining high-quality data. The fnal genome assembly is 177Mbp with contig N50 size of 75kbp and a scafold N50 size of 2.3Mbp. We obtained one of the most complete cestode genome assemblies and annotated 15,169 potential protein-coding genes. The obtained data may help explain cestode gene function and better clarify the evolution of its gene families, and thus the adaptive features evolved during millennia of co-evolution with their hosts.
Issue
1
First Page
302
Last Page
316
DOI
10.1038/s41597-019-0311-3
ISSN
20524463
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Robert M. Nowak, Jan P. Jastrzebski, Wiktor Kusmirek, et al.. "Hybrid de novo whole-genome assembly and annotation of the model tapeworm Hymenolepis diminuta" (2019). Biology Faculty Publications. 18.
https://commons.und.edu/bio-fac/18