John P. Ries

Date of Award


Document Type


Degree Name

Master of Science (MS)




Juglone (5-hydroxy 1:1; napthoquinone), a chemical substance originally isolated from the walnut tree, has not been investigated to any great extent. Preliminary reports revealed that juglone had depressant effects on a number of species of laboratory animals when administered intraperitoneally and was lethal in large doses. The present study was undertaken to elucidate some of the pharmacological properties of juglone when administered intravenously to anesthetized dogs.

Twenty-six adult mongrel dogs were subdivided into three groups. The animals were anesthetized with sodium pentobarbital and infused with the juglone solution through the femoral vein. Two groups of ten each received 3 xng/kg or 5 mg/kg of juglone, respectively, in isotonic glucose. The remaining six animals served as controls and received corresponding volumes of 5% glucose. Upon completion of the infusion, recordings were obtained at half-hour intervals for a period of four hours. The parameters measured were mean blood pressure, hear-t rate, respiration rate, oxygen consumption, hematocrit, electrocardiogram, and body temperature. As a corollary to the project, tissue samples of heart, lung, liver, and kidney were obtained for histopatbological interpretation.

The animals which received 5> mg/kg of juglone exhibited a small increase in mean blood pressure. Both groups of animals receiving juglone developed significant decreases in heart rates at the later time periods. Jurrlone was found to be acutely toxic to dogs, causing atelectasis of the lungs and early degeneration of proximal kidney tubules. The other parameters measured did not change significantly.