Document Type
Article
Publication Date
2-9-2015
Publication Title
International Journal of Nanomedicine
Volume
10
Abstract
Klebsiella pneumoniae (Kp) is one of the most common pathogens in nosocomial infections and is increasingly becoming multiple drug resistant. However, the molecular pathogenesis of Kp in causing tissue injury and dysregulated host defense remains elusive, further dampening the development of novel therapeutic measures. We have previously screened a series of synthetic antimicrobial beta-sheet forming peptides and identified a peptide (IRIKIRIK; ie, IK8L) with a broad range of bactericidal activity and low cytotoxicity in vitro. Here, employing an animal model, we investigated the antibacterial effects of IK8L in acute infection and demonstrated that peritoneal injection of IK8L to mice down-regulated inflammatory cytokines, alleviated lung injury, and importantly, decreased mortality compared to sham-injected controls. In addition, a math model was used to evaluate in vivo imaging data and predict infection progression in infected live animals. Mechanistically, IK8L can kill Kp by inhibiting biofilm formation and modulating production of inflammatory cytokines through the STAT3/JAK signaling both in vitro and in vivo. Collectively, these findings reveal that IK8L may have potential for preventing or treating Kp infection.
First Page
1045
Last Page
1059
DOI
10.2147/IJN.S73303
ISSN
11769114
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 License
Recommended Citation
Shirui Tan, Changpei Gan, Rongpeng Li, et al.. "A Novel Chemosynthetic Peptide with β-Sheet Motif Efficiently Kills Klebsiella pnuemoniae in a Mouse Model" (2015). Chemistry Faculty Publications. 15.
https://commons.und.edu/chem-fac/15