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Cell culture studies routinely seek to monitor cell migration in response to chemoattractant stimuli. Common assays of cell migration employ well inserts and vertical cell migration assessment. This approach does not allow real-time monitoring of cell behavior. To address this need, we sought to develop a horizontal culture platform conducive to time course cell assessment changes in migration, morphology, phenotype etc. Modification of a commercial chamber slide allowed us to quantify cell migration in response to a 20% serum gradient. Based upon this finding, we designed and fabricated a prototype chamber slide for high replicate, real time assessment of cell migration in the serum gradient. The novel chamber slide design was effective for quantifying not only cell migration differences but visualizing cell movement. Optimization of the fabricated design will provide a novel tool for cell biology research.
Biomedical Engineering and Bioengineering
Bittner, Nicholas M.; Nargis, Nelofar; Ifrene, Ghoulem; Voels, Brent Jeffrey; and Combs, Colin K., "Creation of a hyperplane device for horizontal cellular migration assays" (2023). Biomedical Sciences Posters and Presentations. 1.